Device

Part:BBa_K2233002

Designed by: Kyosuke Kita   Group: iGEM17_Kobe   (2017-10-21)


lacZ for monitoring amtB gene expression in B.subtilis chromosome

lacZ and cat (chloramphenicol acetyl transferase) genes flanked by the DNA fragments, which are homologous to the particular regions of B.subtilis (strain NCIB 3610) chromosomal DNA.

This part is designed to insert to the B.subtilis chromosome immediately downstream of amtB gene via homologous recombination. Upon recombination, lacZ can be fused to amtB, allowing the amtB gene expression to be monitored. In this part, lacZ does not include promoter region. Therefore this part has no function by itself unless it is inserted into B.subtilis chromosome. cat gene can be used as a selection marker.

In our project, this part was used in an attempt to measure the concentration of L-theanine by monitoring amtB gene expression in B.subtilis NCIB 3610 chromosome. For more information, please visit our team wiki.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5434
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 592


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Categories
Parameters
None